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Enhanced production of alkaline protease by a mutant of Bacillus licheniformis N-2 for dehairing BABT
Nadeem,Muhammad; Qazi,Javed Iqbal; Baig,Shahjahan.
The purpose of the present investigations was to improve the yield of alkaline protease for leather dehairing by subjecting the indigenous proteolytic strain Bacillus licheniformis N-2 to various mutagenic treatments viz. UV irradiations, NTG (N-methyl-N-nitro-N-nitrosoguinidine) and MMS (methyl methane sulfonate). After screening on skim milk agar plates, a total of nine positive mutants were selected for shake flask experiments. Among these, the best proteolytic mutant designated as UV-9 showed 1.4 fold higher alkaline protease activity in preoptimized growth medium than the parent strain. The fermentation profile and kinetic parameters such u(h-1), Yp/s, Yp/x, Yx/s, q s, Qs, q p and Qp also indicated the superiority of the selected mutant UV-9 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus mutants; Mutagenic treatments; Alkaline protease; Dehairing agent.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500003
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Ethanol production from agricultural wastes using Sacchromyces cervisae BJM
Irfan,Muhammad; Nadeem,Muhammad; Syed,Quratualain.
The main objective of this study was production of ethanol from three lignocellulosic biomasses like sugarcane bagasse, rice straw and wheat straw by Sacchromyces cervisae. All the three substrates were ground to powder form (2 mm) and pretreated with 3%H2O2 + 2% NaOH followed by steaming at 130 °C for 60 min. These substrates were hydrolyzed by commercial cellulase enzyme. The whole fermentation process was carried out in 500 mL Erlenmeyer flask under anaerobic conditions in submerged fermentation at 30 °C for three days of incubation period. FTIR analysis of the substrates indicated significant changes in the alteration of the structure occurred after pretreatment which leads to efficient saccharification. After pretreatment the substrates were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lignoccellulosic biomass; Pretreatment; Sacchromyces cervisae; Ethanol production.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200012
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Kinetics Study of Extracellular Detergent Stable Alkaline Protease from Rhizopus oryzae BABT
Mushtaq,Zareena; Irfan,Muhammad; Nadeem,Muhammad; Naz,Mammona; Syed,Quratulain.
In this study, extracellular alkaline protease was produced from Rhizopus oryzae in submerged fermentation using dairy waste (whey) as a substrate. Fermentation kinetics was studied and various parameters were optimized. The strain produced maximum protease at initial medium pH of 6.0 medium depth of 26 mm, inoculum size of 2% at incubation temperature of 35ºC for 168 h of fermentation. Alkaline protease was purified to homogeneity by ammonium sulphate fractionation followed by sephadex G-100 chromatography. The molecular mass of alkaline protease was 69 kDa determined by 10% SDS-PAGE. The optimum pH and temperature of alkaline protease was 9.0 and 40ºC, respectively. Metal profile of the enzyme showed that the enzyme was non-metallic in nature. The Km ,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kinetics; Purification; Characterization; Protease; Detergent stability; Rhizopus oryzae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200175
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Phytotoxic effect of plant extracts on physiology of cotton (Gossypium hirsutum L.) plants Planta Daninha
N. Zahoor,Muhammad; Nadeem,Muhammad; Iqbal,Jamshaid; F. Shahzad,Muhammad; Islam,Tahir; A. Begum,Hussan; S. Baloch,Mohammad; Ullah,Ayat.
Abstract Background: Plant-derived compounds are alternatives of synthetic insecticides in sustainable agriculture. Objective: This study investigated the phytotoxic effect of higher concentrations (2, 4, 8 and 16%) of four plants extracts (Azadirachta indica, Mentha arvensis, D. stramonium and Citrus limonium) on cotton plants. Methods: Each concentration was replicated four times to check the phytotoxic effect (CO2-in, CO2-out, H2O-in, H2O-out and photosynthesis absorption rate (PAR) in randomized complete block design. Data was recorded after 12, 24, 48 and 72 hours of spray with the help of Photosynthetic CL 340 meter. Results: The results showed that CO2-in was more affected by the D. stramonium (131.65±0.38) at 8% concentration. The overall...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Synthetic insecticides; Plant extract; Cotton; PAR.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100314
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